Coding

Part:BBa_K1957009:Design

Designed by: Nancy Teng   Group: iGEM16_NRP-UEA-Norwich   (2016-10-13)


FeFe Hydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1208


Design Notes

The three BioBricks were originally ligated together in pBAD. This was then extracted out of the pBAD vector and re-inserted into pSB1C3. We suggest that other iGEM teams wanting to use this construct excise the FeFe cluster out of pSB1C3 and ligate it into pBAD.


Source

The source of the part will be its sequence which was retrieved from GenBank.

References